ABSTRACT Desiccation practiced during the preharvest period contributes to mechanized seed harvesting. This work aimed to verify whether wheat preharvest desiccation influences the morphological and physiological characteristics of the seedlings produced from the seeds of desiccated plants. The preharvest treatments included a combination of herbicides (glufosinate-ammonium, glyphosate, and paraquat) and phenological application stages (Zadoks: 83, 85, 87, and 92), as well as a control treatment (without application). Two wheat cultivars were used (BRS Parrudo and TBIO Sinuelo). Herbicide applications were observed to decrease the length and projected area of the shoots by 52 and 46%, respectively, as well as reduce the length, surface area and root volume when compared to the control treatment without application. The hydrogen peroxide concentration, as well as the enzymatic activity of guaiacol peroxidase, was observed to rise only in the treatments where the herbicides were applied. Physiologically, an increased hydrogen peroxide output was revealed, while the guaiacol peroxidase enzymatic activity increased in both cultivars, but with no effect noted in the superoxide dismutase enzymatic activity. The shoot and root morphology were negatively influenced, showing a rise in the enzymatic activity and hydrogen peroxide concentration in the seedlings from the desiccated plants.

ABSTRACT. The objectives of this study were to evaluate the effect of the chemical composition of soybean seeds and the efficiency of seed mineral supplementation on physiological quality and field performance. Two seed lots (high and low levels of mineral nutrition), with or without supplemental macro- and micronutrients, of three soybean cultivars (BMX Potência RR, BMX Turbo RR, and BMX Magna RR) were used. For the laboratory experiment, a completely randomized design with four replications was used. To evaluate the physiological quality of the seeds, the water content, first count, germination, 1,000-seed weight, and length and dry weight of the seedling were determined. For the field experiment, a randomized block design with four replications was used, and seedling emergence and grain yield were evaluated. Seeds with higher nutrient content produced seedlings of greater vigor and higher grain yield for all of the cultivars studied. The use of mineral supplementation in seed lots with low nutrient content resulted in greater vigor under laboratory conditions. The increase in grain yield after mineral supplementation depends on the quality of the seed lot.

Resumo: O objetivo deste trabalho foi avaliar alternativas de manejo do nitrogênio em trigo (Triticum aestivum), com uso da inoculação de Azospirillum brasilense nas sementes e nas folhas. Os tratamentos consistiram da inoculação ou não de A. brasiliense, via sementes ou folhas, associada a fertilizações de cobertura com 0, 70 e 140 kg ha-1 de nitrogênio. Três cultivares de trigo foram testadas: BRS Parrudo, TBIO Quartzo e TBIO Sinuelo. O delineamento experimental utilizado foi em blocos ao acaso, com três repetições. Avaliaram-se as seguintes características: número de plantas emergidas, de perfilhos, de espiguetas por espigas, de grãos por espiga e de grãos por espigueta; massa de mil grãos; massa de hectolitro; e produtividade de grãos. O manejo foliar de A. brasilense apresentou melhor associação com a cultivar TBIO Sinuelo. A aplicação de A. brasilense via foliar, realizada individualmente ou associada ao tratamento de sementes, aumenta a produtividade de grãos e os componentes de produtividade das cultivares avaliadas.

Abstract: The objective of this work was to evaluate nitrogen management alternatives using Azospirillum brasilense inoculation in wheat (Triticum aestivum) by seed and foliar applications. Treatments consisted of the inoculation or not of A. brasilense, via seed and leaves, associated with topdressing with 0, 70, and 140 kg ha-1 nitrogen. Three wheat cultivars were tested: BRS Parrudo, TBIO Quartzo, and TBIO Sinuelo. The experimental design was a randomized complete block, with three replicates. The following traits were evaluated: number of emerged plants, tillers, spikelets per ear, grains per ear, and grains per spikelet; 1,000-grain mass; hectoliter mass; and grain yield. The foliar management of A. brasilense showed a better association with the TBIO Sinuelo cultivar. The foliar application of A. brasilense, whether alone or combined with seed treatment, increases the grain yield and yield components of the evaluated cultivars.

ABSTRACT. Inoculation with nitrogen-fixing bacteria (Bradyrhizobium) is an indispensable technology to increase the productivity of the soybean crop. The objective of this study was to evaluate the viability of the inoculation with Bradyrizobium bacteria associated with the osmoprotector coating on different pre-sowing days and the effect of different soil pH on the components of yield, nodulation and grain yield of soybean. Two soybean cultivars were used, sown in two seasons and submitted to soil pH of 5.3 and 6.5. The seeds were inoculated at different pre-sowin periods with Bradyrizobium bacteria in the presence or absence of osmoprotectants. In the cultivar NA 5909RG (pH 5.3), the seeds inoculated with Bradyrhizobium and osmoprotector, at four and seven days of pre-sowing, presented an increase of 10.8% and 8.3% of productivity, in relation to those without osmoprotector. The osmoprotector and the inoculation of Bradyrhizobium increases the number and dry mass of nodules in soil with more acidic pH. The soil of lower acidity is responsible for higher grain yield, compared to the higher acidity, when both were not inoculated. The variable with the greatest explanatory power and contribution in the variability in the data of the experiment is the grain yield.

RESUMO O incremento na produtividade de grãos pode ser obtido pela interação entre plantas e microrganismos promotores do crescimento vegetal. Bactérias do gênero Bradyrhizobium spp. são capazes de realizar a fixação do nitrogênio em soja [Glycine max (L.) Merril], enquanto as do gênero Azospirillum spp. induzem a síntese de hormônios vegetais. O objetivo do presente trabalho foi avaliar a inoculação com Bradyrhizobium e co-inoculação com Bradyrhizobium + Azospirillum brasilense em sementes de soja associado a aplicação de 0, 75 e 150 kg de N ha-1 de ureia aplicada em cobertura no estádio reprodutivo. Três cultivares de soja (BMX Ativa, TEC 6029 e BMX Potência) foram utilizadas nos experimentos em Santa Maria, RS, Brasil, durante dois anos agrícolas (2013/2014 e 2014/2015) e duas épocas de cultivo. Foram avaliadas as características morfológicas, de nodulação e componentes da produtividade. A co-inoculação proporcionou incremento de 240 kg de grãos ha-1 em relação a inoculação convencional. Quando co-inoculadas, as cultivares BMX Ativa, TEC 6029 e BMX Potência acresceram a produtividade de grãos em 6, 4 e 12% respectivamente. A aplicação de 150 kg de N ha-1 acresceu a produtividade em 300 kg de grãos ha-1, com a co-inoculação das cultivares TEC 6029 e BMX Potência, porém, sem retorno econômico. Os cultivares testados quando inoculados somente com Bradyrhizobium não responderam positivamente à aplicação de ureia.

ABSTRACT Increased grain yield can be obtained via an interaction between plants and growth-promoting microorganisms. The Bradyrhizobium spp. are capable of fixing atmospheric nitrogen in soybeans [Glycine max (L.) Merril], and Azospirillum spp. induce the synthesis of phytohormones. The aim of this study was to evaluate inoculation with Bradyrhizobium and co-inoculation with Bradyrhizobium + Azospirillum brasilense in soybeans in combination with the application a topdressing of 0, 75 or 150 kg of N ha-1 of urea during the reproductive stage. Three soybean cultivars (BMX Ativa, TEC 6029 and BMX Potência), were tested in field experiments in Santa Maria, RS, Brazil, during two agricultural years (2013/2014 and 2014/2015) and two sowing times. Morphological, nodulation and yield components were evaluated. Co-inoculation increased the grain yield by 240 kg ha-1 compared with conventional inoculation. When co-inoculated, cultivars BMX Ativa, TEC 6029 and BMX Potência showed increased grain yields of 6, 4 and 12%, respectively. The application of 150 kg ha-1 of N as a topdressing increased the grain yield by 300 kg ha-1 in the co-inoculated cultivars TEC 6029 and BMX Potência, but without a financial return. When inoculated only with Bradyrhizobium, the cultivars did not respond positively to the application of urea.

RESUMO: O presente experimento teve como objetivo verificar as respostas da aplicação de micronutrientes com reguladores vegetais em tratamento de sementes. O delineamento experimental foi em blocos ao acaso com nove tratamentos e quatro repetições. Os tratamentos foram: 1) Testemunha: sementes sólidas (TS) com 3 % de P2O5, 2 % K2O, 0,17 % Cu, 2,8 % Mn e 6,2 % Zn e uma dose de 100 g ha-1; 2) TS com uma dose de 150 g ha-1; 3) TS com uma dose de 200 g ha-1; 4) Sementes líquidas (TL) com 2 % de P2O5, 1,5 % de K2O, 0,07 % de Cu, 1,2 % de Mn e 2,7 % de Zn a uma dose de 400 mL ha-1; 5) TL, a dose de 500 mL ha-1, TL com uma dose de 600 mL ha-1; 6) regulador de crescimento vegetal que continha citocinina + indol-butilo + ácido giberélico, com classe toxicológica II, 0,09 g L-1 citocinina 0,05 g de álcool L-1 indol-butilo e 0,05 g L-1de ácido giberélico, em uma dose de 500 mL ha-1; 7) um formulado de NPK (08-28-16) 300 kg ha-1. O tratamento composto por aplicação líquida em sementes, 600 mL ha-1, apresentou a maiorers massa seca de parte aérea e radicular, número de grãos e produtividade de grãos.

RESUMEN: El presente experimento fue verificar las respuestas a la aplicación de micronutrientes por la aplicación de tratamiento de semillas y regulador del crecimiento vegetal. El diseño experimental fue de bloques al azar con nueve tratamientos y cuatro repeticiones. Los tratamientos fueron: 1) Testigo: semillas sólido (TS) con 3 % de P2O5, 2 % K2O, 0,17 % Cu, 2,8 % Mn y 6,2 % Zn a una dosis de 100 g ha-1; 2) TS a una dosis de 150 g ha-1; 3) TS a una dosis de 200 g ha-1; 4) semillas líquido (TL) con 2 % de P2O5, 1,5 % de K2O, 0,07 % de Cu, 1,2 % de Mn y 2,7 % de Zn a una dosis de 400 mL ha-1; 5) TL, dosis de 500 ml ha-1, TL a una dosis de 600 mL ha-1; 6) regulador del crecimiento vegetal que tiene citoquinina + indol-butilo + ácido giberélico, posee clase toxicológica II, 0,09 g L-1 citoquinina 0,05 g de alcohol L-1 indol-butilo y 0,05 g L-1de ácido giberélico, en una dosis de 500 mL ha-1; 7) un formulado NPK (08-28-16) 300 kg ha-1. El tratamiento de semillas, ya sea en forma sólida o líquida, promueve un aumento en la acumulación de materia seca en la parte aérea y raíces, así como en el número de granos y en el rendimiento de granos de maíz, siendo la dosis de 600 mL ha-1 de la que promueve las mejores productividades.

O objetivo do presente trabalho foi identificar a diversidade da micobiota parasitária e o tempo decorrido até o início do parasitismo de escleródios de Sclerotinia sclerotiorum em solos da fronteira oeste do Rio Grande do Sul (RS). Foram coletados solos agrícolas e não agrícolas de cinco localidades na fronteira oeste do Rio Grande do Sul. O isolamento dos fungos foi realizado por meio do teste de iscas, sendo que as iscas foram os escleródios do fitopatógeno, os quais permaneceram enterrados no solo durante 15, 30 e 60 dias. Os fungos foram identificados em nível de gênero. Os gêneros fúngicos encontrados parasitando escleródios foram: Trichoderma, Penicillium, Aspergillus, Fusarium, Pythium(Cromista), Clonostachys rosea (Gliocladium), Chaetomium, Curvularia, Phytophthora(Cromista), Rhizopus, Lichtheimia (Absidia), Mucor, Acremonium, Periconiae Isaria.Observou-se que os escleródios foram parasitados pelos fungos até 15 dias depois de serem enterrados, e deste período em diante apenas a frequência de ocorrência dos fungos mudou. Concluiu-se que os gêneros fúngicos Isaria,Periconia, Acremonium, Lichtheimia (Absidia), Phytophthora, Curvulariae Chaetomium são encontrados em solos agrícolas e não agrícolas na fronteira oeste do RS, e não foram citados anteriormente em outros trabalhos. Existe diversidade de fungos parasitários de escleródios de S. sclerotiorum nos solos da fronteira oeste do RS, os quais podem ser utilizados em experimentos para compor um programa de controle biológico desse fitopatógeno. Os escleródios são parasitados pelos fungos durante até 15 dias, após os mesmos serem enterrados no solo.

The aim of this study was to identify the diversity of the parasitic mycoflora and to identify the necessary time until the beginning of the parasitism of sclerotia of Sclerotinia sclerotiorum in soils of the western frontier of the state of Rio Grande do Sul (RS), Brazil. Agricultural and non agricultural soils of five locations in the western frontier of Rio Grande do Sul were collected. The fungi isolation was performed by testing baits, and baits were the sclerotia of the phytopathogen, which remained buried in the soil for 15, 30 and 60 days. The fungi were identified at the genus level. The following fungal genera were found parasitizing sclerotia: Trichoderma, Penicillium, Aspergillus, Fusarium, Pythium (Cromista) Clonostachys rosea (Gliocladium), Chaetomium, Curvularia, Phytophthora (Cromista), Rhizopus, Lichtheimia (Absidia), Mucor, Acremonium, Periconiaand Isaria. It was observed that the sclerotia were parasitized by fungi until 15 days after they had been buried. And after this period, only the frequency of occurrence of fungi has changed. It was concluded that the fungal genera Isaria, Periconia, Acremonium, Lichtheimia (Absidia), Phytophthora, Curvulariaand Chaetomium are found both in agricultural and non-agricultural soils on the western frontier of RS, but they were not previously cited in other works. There is diversity of parasitic fungi of sclerotia of S. sclerotiorum in the soils of the western frontier of the RS, which can be used in experiments to compose biological control programs for this pathogen. The sclerotia are parasitized by fungi until 15 days after being buried underground.